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Bovine serum albumin observed by infrared spectrometry. II. Hydration mechanisms and interaction configurations of embedded H 2 O molecules
Author(s) -
Grdadolnik Joze,
Maréchal Yves
Publication year - 2000
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/1097-0282(2001)62:1<54::aid-bip70>3.0.co;2-4
Subject(s) - chemistry , steric effects , molecule , hydrogen bond , amide , infrared spectroscopy , bovine serum albumin , crystallography , computational chemistry , stereochemistry , organic chemistry , chromatography
The hydration mechanism of bovine serum albumin (BSA) is studied, and we analyze (de)hydration spectra displayed previously. We first determine the three elementary (de)hydration spectra on which all these (de)hydration spectra can be decomposed. They correspond to three different hydration mechanisms for the protein, which we define after a quantitative analysis performed in a second step. The first mechanism, which involves ionization of carboxylic COOH groups, occurs at low hydration levels and rapidly reaches a plateau when the hygroscopy is increased. It is a mechanism that involves a single H 2 O molecule and consequently requires somewhat severe steric conditions. The second mechanism occurs at all hydration levels and, because it involves more H 2 O molecules, requires less severe steric conditions. It consists of the simultaneous hydration of one amide NH group and one carbonyl‐amide CO group by four H 2 O molecules and one carboxyl COO − group by eight H 2 O molecules. The third mechanism is simpler and consists of the introduction of H 2 O molecules into the hydrogen‐bond network of the hydrated protein. It becomes important at a high hydration level, when the presence of an appreciable number of H 2 O molecules makes this hydrogen‐bond network well developed. This analysis also shows that 80 H 2 O molecules remain embedded in one dried protein made of 604 peptide units. They are held by hydrogen bonds established by NH groups and at the same time they establish two hydrogen bonds on two carbonyl‐amide CO groups. The proportion of free NH groups can be determined together with that of carbonyl‐amide CO groups accepting no hydrogen bonds and that of carbonyl‐amide CO groups accepting two hydrogen bonds. The proportion of NH groups establishing one hydrogen bond directly on a carbonyl‐amide CO group is 65%, which is the proportion of peptide units found in α helices in BSA. © 2000 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 62: 54–67, 2001