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The synthesis of phosphopeptides
Author(s) -
McMurray John S.,
Coleman David R.,
Wang Wei,
Campbell Martin L.
Publication year - 2001
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/1097-0282(2001)60:1<3::aid-bip1001>3.0.co;2-l
Subject(s) - chemistry , dephosphorylation , phosphopeptide , phosphorylation , protein phosphorylation , sh2 domain , phosphatase , transcription factor , biochemistry , phosphoprotein , antennapedia , signal transduction , microbiology and biotechnology , computational biology , protein tyrosine phosphatase , protein kinase a , homeobox , gene , biology
Phosphorylation and dephosphorylation are key events in protein expression and regulation and in signal transduction. Phosphopeptides are very useful reagents for the study of these processes, and have been used to great advantage in the study of phosphatase substrate specificity, SH2 domain ligand specificity, and protein–protein interactions. Furthermore, the advent of cell‐permeable peptide carriers, such as those from the antennapedia homeodomain and the HIV TAT transcription factor, has allowed the study of intracellular events, thus underscoring the utility of these reagents. In this paper we review methods for the synthesis of phosphopeptides with the emphasis on the preparation of phosphoamino acid building blocks. © 2001 John Wiley & Sons, Inc. Biopolymers (Pept Sci) 60: 3–31, 2001