Premium
Characterization of substance P–membrane interaction by transferred nuclear Overhauser effect
Author(s) -
Augé S.,
Bersch B.,
Tropis M.,
Milon A.
Publication year - 2000
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/1097-0282(20001015)54:5<297::aid-bip10>3.0.co;2-9
Subject(s) - chemistry , nuclear overhauser effect , nuclear magnetic resonance spectroscopy , phosphatidylcholine , helix (gastropod) , stereochemistry , vesicle , substance p , peptide , two dimensional nuclear magnetic resonance spectroscopy , membrane , crystallography , receptor , biochemistry , neuropeptide , phospholipid , ecology , snail , biology
Substance P, one of the mammalian tachykinins, is known to interact strongly with lipid bilayers and this interaction may play a role in the receptor–peptide recognition process. The conformation of substance P bound to vesicles consisting of perdeuterated phosphatidylcholine has been investigated by means of two‐dimensional transferred nuclear Overhauser (trNOE) spectroscopy. Nuclear magnetic resonance data analysis resulted in a unique conformational family characterized by a well‐defined conformation of the last seven C‐terminal amino acids, which consists of a sequence of nonstandard turns following each other in a helix‐like manner. The absence of short‐ or medium‐range trNOE in the N‐terminal part indicates its structural flexibility. © 2000 John Wiley & Sons, Inc. Biopoly 54: 297–306, 2000