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Quantitation of aliphatic suberin in Quercus suber L. cork by FTIR spectroscopy and solid‐state 13 C‐NMR spectroscopy
Author(s) -
Lopes M. H.,
Neto C. Pascoal,
Barros A. S.,
Rutledge D.,
Delgadillo I.,
Gil A. M.
Publication year - 2000
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/1097-0282(2000)57:6<344::aid-bip40>3.0.co;2-#
Subject(s) - quercus suber , suberin , cork , chemistry , fourier transform infrared spectroscopy , spectroscopy , nuclear magnetic resonance spectroscopy , solid state , analytical chemistry (journal) , organic chemistry , lignin , chemical engineering , biochemistry , physics , quantum mechanics , engineering
This work determined that the percentage of suberin in cork may be found by solid‐state 13 C cross polarization/magic angle spinning (CP/MAS) NMR spectroscopy and by FTIR with photoacoustic detection (FTIR‐PAS) spectroscopy. A linear relationship is found between the suberin content measured through CP/MAS spectral areas and that measured gravimetrically. Furthermore, application of a partial least squares (PLS1) regression model to the NMR and gravimetric data sets clearly correlates the two sets, enabling suberin quantification with 90% precision. Suberin quantitation by FTIR‐PAS spectroscopy is also achieved by a PLS1 regression model, giving 90% accurate estimates of the percentage of suberin in cork. Therefore, 13 C‐CP/MAS NMR and FTIR‐PAS proved to be useful and accurate noninvasive techniques to quantify suberin in cork, thus avoiding the traditional time consuming and destructive chemical methods. © 2000 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 57: 344–351, 2000