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FTIR spectroscopic and HPLC chromatographic studies of carbon tetrachloride induced acute hepatitis in rats: Damage in liver phospholipid membrane
Author(s) -
Yoon Seokjoo,
Kazusaka Akio,
Fujita Shoichi
Publication year - 2000
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/1097-0282(2000)57:5<267::aid-bip30>3.0.co;2-u
Subject(s) - carbon tetrachloride , chemistry , microsome , phosphatidylethanolamine , phospholipid , phosphatidylcholine , chromatography , diacylglycerol kinase , chloroform , membrane , high performance liquid chromatography , intraperitoneal injection , lipid peroxidation , ccl4 , biochemistry , antioxidant , organic chemistry , pharmacology , medicine , protein kinase c , enzyme
Carbon tetrachloride (CCl 4 ) induced rat hepatitis was studied by observing an FTIR spectrum of the liver microsomal or homogenate extract compared with those of model compounds. The microsomal extract from the liver of healthy control rats showed almost the same spectrum as a mixture of phosphatidylcholine and phosphatidylethanolamine (2:1 by weight). Intraperitoneal injection of CCl 4 decreased the absorption intensity due to the CH in the CCH at 3012 cm −1 in the microsomal extract, and it developed a new 1,2‐diacylglycerol band at 1070 cm −1 in the homogenate extract. An HPLC study was added to assign the 1070 cm −1 band to 1,2‐diacylglycerol. These findings were interpreted from the peroxidation of the microsomal membrane and the regenerative proliferation of the damaged cell. © 2000 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 57: 267–271, 2000