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Nonionic amphiphilic polymers derived from Tris(hydroxymethyl)‐acrylamidomethane keep membrane proteins soluble and native in the absence of detergent
Author(s) -
Prata C.,
Giusti F.,
Gohon Y.,
Pucci B.,
Popot J.L.,
Tribet C.
Publication year - 2000
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/1097-0282(2000)56:2<77::aid-bip1053>3.0.co;2-b
Subject(s) - chemistry , hydroxymethyl , tris , bacteriorhodopsin , amphiphile , telomerization , polymer , polymer chemistry , micelle , membrane , organic chemistry , chromatography , biochemistry , aqueous solution , copolymer , catalysis
A new family of amphipols—amphiphilic polymers designed to form water‐soluble complexes with membrane proteins—was synthesized by free‐radical telomerization of Tris(hydroxymethyl)‐acrylamidomethane (THAM) and derivatized THAM. Some of these polymers were found to prevent aggregation and denaturation of two model membrane proteins, bacteriorhodopsin and cytochrome b 6 f, in the absence of detergent micelles. © 2001 John Wiley & Sons, Inc. Biopolymers 56: 77–84, 2001