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Coherent membrane supports for parallel microsynthesis and screening of bioactive peptides
Author(s) -
Wenschuh Holger,
VolkmerEngert Rudolf,
Schmidt Margit,
Schulz Marco,
SchneiderMergener Jens,
Reineke Ulrich
Publication year - 2000
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/1097-0282(2000)55:3<188::aid-bip20>3.0.co;2-t
Subject(s) - linker , chemistry , combinatorial chemistry , nanotechnology , scope (computer science) , high throughput screening , computer science , computational biology , materials science , biochemistry , biology , programming language , operating system
Since its invention the SPOT‐synthesis methodology has become one of the most efficient strategies for the miniaturized assembly of large numbers of peptides. The combination of a facile synthetic method with high throughput solid‐ and solution‐phase screening assays qualifies the SPOT‐technique as a valuable tool in biomedical research. Recent developments such as the introduction of novel polymeric surfaces, new linker and cleavage strategies as well as automated robot systems extended the scope of practical chemical reactions that can be accommodated as well as the numbers of compounds obtainable by this technique. Thus, highly complex spatially addressed compound arrays have become accessible. Together with the introduction of novel screening assays, the method is excellently suited to elucidate recognition events on the molecular level. © 2000 John Wiley & Sons, Inc. Biopolymers (Pept Sci) 55: 188–206, 2000