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Analysis of triacylglycerols with non‐aqueous reversed‐phase liquid chromatography and positive ion electrospray tandem mass spectrometry
Author(s) -
Hvattum Erlend
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20010215)15:3<187::aid-rcm211>3.0.co;2-t
Subject(s) - chemistry , electrospray , mass spectrometry , tandem mass spectrometry , chromatography , electrospray ionization , aqueous solution , collision induced dissociation , ion , formate , dissociation (chemistry) , chloroform , analytical chemistry (journal) , fatty acid , organic chemistry , catalysis
A non‐aqueous reversed‐phase liquid chromatographic method coupled to electrospray ionisation (ESI) tandem mass spectrometry was developed for the analysis of triacylglycerols (TGs). The synthetic TGs studied were separated according to their equivalent carbon number with a gradient of methanol (containing 0.01% (v/v) formate adjusted to pH 5.3 with ammonia) and chloroform. ESI mass spectra of TGs yielded positive ion current signals for [M + NH 4 ] + and [M + NH 4 −RCOONH 4 ] + . The mass spectra also showed signals believed to arise from [M + K] + . Collision‐induced dissociation (CID) of the [M + NH 4 ] + precursor ion yielded [M + NH 4 − RCOONH 4 ] + , [RCO + 74] + and [RCO] + product ions as aids for the structural elucidation of the TGs. In addition, [RCO − 18] + and small amounts of [RCO − 2] + product ions were also found. The latter ions were observed only for TGs containing unsaturated fatty acids. CID of ammoniated 1‐stearoyl‐2‐oleoyl‐3‐linoleoyl‐glycerol (18:0/18:1/18:2) indicated that neutral loss of the sn ‐2 fatty acid was energetically less favourable than loss of the fatty acid from the sn ‐1 or sn ‐3 position. Copyright © 2001 John Wiley & Sons, Ltd.