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Validation of liquid‐liquid extraction followed by flow‐injection negative ion electrospray mass spectrometry assay to Topiramate in human plasma
Author(s) -
Chen Su,
Carvey Paul M.
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20010130)15:2<159::aid-rcm210>3.0.co;2-w
Subject(s) - chemistry , topiramate , chromatography , mass spectrometry , electrospray , electrospray ionization , calibration curve , extraction (chemistry) , tandem mass spectrometry , detection limit , selected reaction monitoring , liquid chromatography–mass spectrometry , neuroscience , epilepsy , biology
This paper describes the development and validation of a method for the quantitative analysis of Topiramate (2,3:4,5‐bis‐ O ‐(1‐methylethylidene)‐β‐ D ‐fructopyranose sulfamate), a new antiepileptic drug, in human plasma using liquid‐liquid extraction followed by flow‐injection negative ion electrospray mass spectrometry. Using Prednisone (1,4‐pregnadiene‐17‐α,21‐diol‐3,11,20‐trione [10 µg/mL]) as an internal standard, calibration curves for Topiramate were linear over a range of 1 to 30 µg/mL in human plasma and were highly reliable ( r 2 = 0.9991). The lower limit of quantitation of the assay was 2 µg/mL in human plasma. Precision (%CV <15%) and accuracy (<20%) for both intra‐ and inter‐day validations were satisfactory. The method has been used in clinical pharmacology research. Copyright © 2001 John Wiley & Sons, Ltd.