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Ultrafast liquid chromatography/ultraviolet and liquid chromatography/tandem mass spectrometric analysis
Author(s) -
Cheng YungFong,
Lu Ziling,
Neue Uwe
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20010130)15:2<141::aid-rcm201>3.0.co;2-i
Subject(s) - chemistry , chromatography , electrospray , analyte , selectivity , tandem , tandem mass spectrometry , mass spectrometry , liquid chromatography–mass spectrometry , high performance liquid chromatography , analytical chemistry (journal) , organic chemistry , materials science , composite material , catalysis
Optimal liquid chromatography/mass spectrometric [LC/MS(/MS)] analysis depends on both the LC selectivity and the electrospray efficiency. Here, we outline a simple and comprehensive LC/MS/MS strategy for the rapid analysis of a wide range of pharmaceutical compounds. To achieve ultrafast LC separation with little sacrifice in peak capacity, one needs to start with a column that provides a good peak capacity at short gradient run times; secondly, it is important to use high flow rates to achieve a good gradient peak capacity. Following this strategy, it was possible to baseline‐resolve a mixture (containing acidic, neutral, and basic pharmaceutical analytes) in seconds. By coupling the selectivity provided by fast LC separation with the specificity of MS/MS detection, it is possible to separate and identify a wide range of analytes in 1‐min gradient analyses. Also, the impact of mobile phase pH on both the chromatographic selectivity and the MS/MS sensitivity is demonstrated. Copyright © 2001 John Wiley & Sons, Ltd.