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Determination of γ‐hydroxybutyric acid (GHB) in plasma and urine by headspace solid‐phase microextraction and gas chromatography/positive ion chemical ionization mass spectrometry
Author(s) -
Frison Giampietro,
Tedeschi Luciano,
Maietti Sergio,
Ferrara Santo Davide
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20001230)14:24<2401::aid-rcm179>3.0.co;2-i
Subject(s) - chemistry , chromatography , mass spectrometry , urine , gas chromatography–mass spectrometry , chemical ionization , selected ion monitoring , gas chromatography , solid phase microextraction , ionization , ion , biochemistry , organic chemistry
A new method for the qualitative and quantitative analysis of γ‐hydroxybutyric acid (GHB) in plasma and urine samples is described. It involves the conversion of GHB to γ‐butyrolactone (GBL), its subsequent headspace solid‐phase microextraction (SPME), and detection by gas chromatography/positive ion chemical ionization mass spectrometry (GC/PICI‐MS), using D 6 ‐GBL as internal standard. The assay is linear over a plasma GHB range of 1–100 µg/mL (n = 5, r = 0.999) and a urine GHB range of 5–150 µg/mL (n = 5, r = 0.998). Relative intra‐ and inter‐assay standard deviations, determined for plasma and urine samples at 5 and 50 µg/mL, are all below 5%. The method is simple, specific and reasonably fast. It may be applied for clinical and forensic toxicology as well as for purposes of therapeutic drug monitoring. Copyright © 2000 John Wiley & Sons, Ltd.