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Characterization of Aspergillus spores by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Li TzuYing,
Liu BiingHui,
Chen YuChie
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20001230)14:24<2393::aid-rcm178>3.0.co;2-9
Subject(s) - chemistry , spore , mass spectrometry , desorption , matrix (chemical analysis) , chromatography , matrix assisted laser desorption/ionization , mass spectrum , aspergillus flavus , analytical chemistry (journal) , microbiology and biotechnology , food science , organic chemistry , adsorption , biology
Abstract The intact fungal spores of several strains of four Aspergillus species, Aspergillus flavus, A. oryzae, A. parasiticus , and A. sojae , were directly analyzed by matrix‐assisted laser desorption/ionization (MALDI) time‐of‐flight mass spectrometry. Very simple MALDI mass spectra are obtained by directly mixing spores with a matrix such as α‐cyano‐4‐hydroxycinnamic acid or sinapinic acid. The mass spectra are obtained from the ablation of cell walls of spores owing to the acidity of the matrix solution. The MALDI results show that aflatoxigenic strains and non‐aflatoxigenic strains have different mass peak profiles. Furthermore, the MALDI results of non‐aflatoxigenic A. flavus and A. parasiticus spores resemble those of the closely related A. oryzae and A. sojae spores, respectively. Copyright © 2000 John Wiley & Sons, Ltd.