Premium
Detection of exogenous intake of natural corticosteroids by gas chromatography/combustion/isotope ratio mass spectrometry: application to misuse in sport
Author(s) -
Bourgogne Emmanuel,
Herrou Valérie,
Mathurin JeanCharles,
Becchi Michel,
Ceaurriz Jacques de
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20001230)14:24<2343::aid-rcm171>3.0.co;2-z
Subject(s) - chemistry , isotope ratio mass spectrometry , chromatography , mass spectrometry , cortisone , gas chromatography–mass spectrometry , urine , gas chromatography , combustion , extraction (chemistry) , corticosteroid , organic chemistry , medicine , biochemistry
A detailed procedure for the analysis of exogenous hydrocortisone and cortisone in urine by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) is proposed. As urinary levels of hydrocortisone are rather low for GC/C/IRMS analysis, the focus is on the main corticosteroid metabolites, tetrahydrocortisone (THE) and tetrahydrocortisol (THF). Following different solid phase extraction purifications, THE and THF are oxidized to 5β‐androstanetrione before analysis by GC/C/IRMS. Significant differences in δ 13 C‰ values of synthetic natural corticosteroids and endogenous human corticosteroids have been observed. Therefore, a positive criterion, to detect exogenous administration of synthetic corticosteroids in anti‐doping control, is proposed. Copyright © 2000 John Wiley & Sons, Ltd.