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Characterization of methylation site of monomethylflavan‐3‐ols by liquid chromatography/electrospray ionization tandem mass spectrometry
Author(s) -
CrenOlivé Cécile,
Déprez Stéphanie,
Lebrun Stéphane,
Coddeville Bernadette,
Rolando Christian
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20001215)14:23<2312::aid-rcm160>3.0.co;2-a
Subject(s) - chemistry , electrospray ionization , fragmentation (computing) , tandem mass spectrometry , chromatography , electrospray , catechin , mass spectrometry , protein mass spectrometry , extractive electrospray ionization , tandem , liquid chromatography–mass spectrometry , sample preparation in mass spectrometry , organic chemistry , polyphenol , materials science , computer science , composite material , antioxidant , operating system
Liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) was used for the structural characterization and differentiation of four isomeric O ‐monomethylated catechins (on phenolic positions) by the analysis of the fragmentation behaviour of catechin. The catechin fragmentation routes were rationalized and it is shown that several diagnostic ions such as 1,3 A + , 1,2 B + , and 1,4 B + allow the unambiguous identification of the methylated ring. The precise position of the methyl group on each ring is determined by the difference in the relative intensities of the diagnostic ions. Isomeric O ‐methylepicatechins were also differentiated using this methodology. Copyright © 2000 John Wiley & Sons, Ltd.