Premium
Analysis of some commercial polysorbate formulations using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Ayorinde Folahan. O.,
Gelain Slair V.,
Johnson James. H.,
Wan Lily W.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20001130)14:22<2116::aid-rcm142>3.0.co;2-1
Subject(s) - chemistry , polysorbate , chromatography , polyvinyl alcohol , polyethylene glycol , sorbitan , mass spectrometry , organic chemistry , pulmonary surfactant , biochemistry , fatty acid , fatty acid ester
Analyses of polysorbate formulations (Tween®20, Tween®40, Tween®60, and Tween®80) by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) reveal a complex mixture of oligomers that include polyethylene glycols, polyethylene glycol esters, isosorbide polyethoxylates, sorbitan polyethoxylates, polysorbate monoesters, polysorbate diesters, and sorbitol polyethoxylate esters. The MALDI‐TOF mass spectra for these formulations show the presence of sodiated molecules in which the major signals are attributed to the presence of polyethylene glycols, isosorbide polyethoxylates, and sorbitan polyethoxylates. Additionally, the complexity of the spectra was correlated to the constituent fatty acid moieties in the polysorbate formulations. Thus Tween®20 showed the presence of polysorbate monolaurates, polysorbate monomyristates, and polysorbate monopalmitates. Tween®40 contained polysorbate mono‐ and dipalmitates. Tween®60 contained polysorbate monopalmitates and polysorbate monostearates. For the Tween®80, mass assignment for polysorbate monooleates and polysorbate dioleates was equivocal, because both of these oligomeric series have the same molecular weight as the sorbitan polyethoxylates, and thus the Tween®80 MALDI‐TOF spectrum appeared to be the least complicated of the four commercial polysorbate formulations. Copyright © 2000 John Wiley & Sons, Ltd.