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Characterization of amiodarone metabolites and impurities using liquid chromatography/atmospheric pressure chemical ionization mass spectrometry
Author(s) -
Myung SeungWoon,
Chang YoonJung,
Min HyeKi,
Kim DongHyun,
Kim Myungsoo,
Kang TaeKyung,
Yoo EunAh,
Sohn Young Taek,
Yim YongHyeon
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20001115)14:21<2046::aid-rcm132>3.0.co;2-4
Subject(s) - chemistry , atmospheric pressure chemical ionization , chromatography , mass spectrometry , impurity , chemical ionization , liquid chromatography–mass spectrometry , high performance liquid chromatography , metabolite , tandem mass spectrometry , thermospray , ionization , selected reaction monitoring , biochemistry , ion , organic chemistry
Using the high performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/APCI‐MS/MS) technique, together with established trends from the literature, the structures of metabolites and impurities of amiodarone, an anti‐arhythmic drug, have been assigned. By comparing analyses of products of incubation with rat liver microsomes with controls in which glucose 6‐phosphate dehydrogenase was omitted, metabolites could be distinguished from impurities. Structures for the two proposed metabolites and four impurities are proposed. Copyright © 2000 John Wiley & Sons, Ltd.