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Analysis of metal complex azo dyes by high‐performance liquid chromatography/electrospray ionization mass spectrometry and multistage mass spectrometry
Author(s) -
Lemr Karel,
Holc̆apek Michal,
Jandera Pavel,
Lyc̆ka Antonín
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20001030)14:20<1881::aid-rcm107>3.0.co;2-i
Subject(s) - chemistry , mass spectrometry , electrospray ionization , fragmentation (computing) , collision induced dissociation , electrospray , mass spectrum , chromatography , acetonitrile , aqueous solution , dissociation (chemistry) , sample preparation in mass spectrometry , analytical chemistry (journal) , ammonium acetate , high performance liquid chromatography , fourier transform ion cyclotron resonance , tandem mass spectrometry , organic chemistry , computer science , operating system
Five metal complex azo compounds were analyzed using negative‐ion electrospray ionization mass spectrometry (ESI‐MS). Mass spectra of all compounds yield intense peaks corresponding to [M — H] − ions without any fragmentation, where M denotes the neutral compound with a proton as the counterion. Under collision induced dissociation (CID) conditions, structurally important fragment ions were studied using the ion trap analyzer with a multistage mass spectrometry (MS n facility. Synthesized compounds with 15 N atoms in the azo group facilitated the fragmentation pattern recognition. A reversed‐phase high‐performance liquid chromatography (HPLC) method using 5 mM ammonium acetate in 70% aqueous acetonitrile as mobile phase was developed making possible the separation of all complex compounds tested. The lower detection limits of the ESI‐MS method are in the range 10–20 ng of each compound. The HPLC/ESI‐MS method makes possible the monitoring of ligand exchange in aqueous solutions of metal complex azo dyes, and also investigation of the stabilities of the complexes in solution. Copyright © 2000 John Wiley & Sons, Ltd.