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Matrix‐assisted laser desorption/ionization time‐of‐flight analysis of Bacillus spores using a 2.94 µm infrared laser
Author(s) -
Ryzhov Victor,
Bundy Jonathan L.,
Fenselau Catherine,
Taranenko Nelli,
Doroshenko Vladimir,
Prasad Coorg R.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20000930)14:18<1701::aid-rcm82>3.0.co;2-i
Subject(s) - chemistry , ionization , laser , matrix (chemical analysis) , desorption , infrared , spore , analytical chemistry (journal) , mass spectrometry , chromatography , optics , organic chemistry , ion , botany , adsorption , biology , physics
The performance of infrared (2.94 µm) and ultraviolet (337 nm) lasers were compared for analysis of purified spores of B. subtilis, B. cereus and B. globigii on a four‐inch end‐cap reflectron time‐of‐flight instrument. Infrared matrix‐assisted laser desorption/ionization (IR‐MALDI) mass spectra of these microorganisms displayed a larger number of biomarker peaks above m/z 4000, compared with UV‐MALDI. Biomarker peaks were observed at higher m/z values with the IR laser. Copyright © 2000 John Wiley & Sons, Ltd.