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Determination of 2‐substituted benzothiazoles of industrial use from water by liquid chromatography/electrospray ionization tandem mass spectrometry
Author(s) -
Reemtsma Thorsten
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20000915)14:17<1612::aid-rcm70>3.0.co;2-c
Subject(s) - chemistry , chromatography , electrospray ionization , direct electron ionization liquid chromatography–mass spectrometry interface , liquid chromatography–mass spectrometry , extractive electrospray ionization , tandem mass spectrometry , mass spectrometry , electrospray , sample preparation in mass spectrometry , protein mass spectrometry , tandem , ionization , chemical ionization , organic chemistry , ion , materials science , composite material
A method was developed for the analysis of 2‐substituted benzothiazoles from wastewaters by reversed‐phase liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS). While benzothiazole (BT), 2‐amino‐ (ABT), 2‐methyl‐, 2‐thiocyanomethylthio‐ and 2‐methylthiobenzothiazole were separated with a methanol gradient acidified with formic acid and detected in the positive mode, 2‐mercapto‐ and 2‐hydroxybenzothiazole (OHBT) as well as benzothiazole‐2‐sulfonic acid (BTSA) were separated with the addition of ammonium acetate and detected in the negative ion mode. Detection limits range from 10 ng L −1 for ABT to 200 ng L −1 for OHBT after direct injection of 100 µL sample volumes, while BT requires 2.5 µg L −1 . The method is, thus, suitable for direct analysis in wastewaters. Its application to tannery effluent samples revealed, that, besides a 90–95% elimination of total benzothiazoles, the concentration of OHBT and BTSA increased during the biological treatment. Copyright © 2000 John Wiley & Sons, Ltd.