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Pyrolysis/gas chromatography/mass spectrometry monitoring of fungal‐biotreated distillery wastewater using Trametes sp. I‐62 (CECT 20197)
Author(s) -
González Tania,
Terrón María C.,
Yagüe Susana,
Zapico Ernesto,
Galletti Guido C.,
González Aldo E.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20000815)14:15<1417::aid-rcm41>3.0.co;2-i
Subject(s) - chemistry , vinasse , effluent , melanoidin , pyrolysis , bagasse , laccase , gas chromatography , chromatography , wastewater , mass spectrometry , chemical oxygen demand , fermentation , manganese peroxidase , pyrolysis–gas chromatography–mass spectrometry , pulp and paper industry , environmental chemistry , food science , organic chemistry , waste management , maillard reaction , engineering , enzyme
Distillery wastewaters generated by ethanol production from fermentation of sugar‐cane molasses, named vinasses, lead to important ecological impact due to their high content of soluble organic matter and their intense dark‐brown color. Taking advantage of the well‐known ability of white‐rot fungi to degrade an extensive variety of organic pollutants, the capacity of Trametes sp. I‐62 (CECT 20197) to detoxify this type of effluents was evaluated. In this work, pyrolysis/gas chromatography/mass spectrometry was applied to the chemical characterization of several fractions of Cuban distillery wastewater as well as to monitoring the changes which occurred after fungal treatment with this white‐rot basidiomycete. Maximum effluent decolorization values and chemical oxygen demand reduction attained after seven days of fungal treatment were 73.3 and 61.7%, respectively, when 20% (v/v) of distillery vinasses was added to the culture medium. Under these conditions a 35‐fold increase in laccase production by Trametes sp. I‐62 was measured, but no manganese peroxidase activity could be detected. The pyrolysis/gas chromatography/mass spectrometry results showed a decrease in a number of pyrolysis products after seven days of fungal treatment, mainly furan derivatives. The decrease in the relative areas of these compounds could be related to the vinasse color‐removal associated with melanoidin degradation. All these results indicated the potential use of Trametes sp. I‐62 in the detoxification of recalcitrant distillery vinasses. Copyright © 2000 John Wiley & Sons, Ltd.