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Interpretation of electrospray/ion trap mass spectra of bile acids and other surfactants
Author(s) -
Rodríguez M. Alejandra,
Yost Richard A.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20000815)14:15<1398::aid-rcm37>3.0.co;2-e
Subject(s) - chemistry , electrospray , ion , mass spectrum , ion trap , analytical chemistry (journal) , chromatography , mass spectrometry , bile acid , spectral line , micelle , molecule , aqueous solution , organic chemistry , biochemistry , physics , astronomy
Electrospray spectra of various bile acids and other surfactants were obtained using an ion trap instrument. Bile acids and bile acid derivatives such as 3‐[(3‐cholamidopropyl)dimethylammonio]‐1‐propanesulfonate (CHAPS) self associate to form micelles in a stepwise process. Their spectra show a distinct pattern of aggregation, with ions evenly separated in the m/z space. A new parameter, n/z , can be used to characterize such ions, where n is the number of molecules and z is the charge of the aggregate. The values of z were determined using multi‐stage mass spectrometry (MS n ) and high resolution in the ion trap. Copyright © 2000 John Wiley & Sons, Ltd.