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Identification of proteins from one‐dimensional sodium dodecyl sulfate‐polyacrylamide gel electrophoresis using electrospray quadrupole‐time‐of‐flight tandem mass spectrometry
Author(s) -
Marvin Laure,
Millar Allan,
Saulot Vincent,
Machour Nadine,
Charlionet Roland,
Tron Francois,
Lange Catherine
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20000730)14:14<1287::aid-rcm27>3.0.co;2-2
Subject(s) - chemistry , chromatography , quadrupole time of flight , mass spectrometry , tandem mass spectrometry , protein mass spectrometry , top down proteomics , polyacrylamide gel electrophoresis , gel electrophoresis , electrospray , tandem , electrospray ionization , sodium dodecyl sulfate , bottom up proteomics , biochemistry , enzyme , materials science , composite material
The potential of electrospray tandem mass spectrometry using a quadrupole‐time‐of‐flight tandem mass spectrometer was evaluated for the identification of two unknown proteins from one‐dimensional polyacrylamide gel electrophoresis (1‐D‐PAGE). Two proteins from cellular cultures of mammary epithelia were purified by 1D‐PAGE. Their identification was achieved using peptide sequence tags generated by LC/Q‐TOF‐MS/MS, whereas MALDI‐TOF mass mapping failed for these proteins obtained from simple 1D‐PAGE separation. Copyright © 2000 John Wiley & Sons, Ltd.