Premium
A simple sample preparation for enhancing the sensitivity of mass spectrometric oligosaccharide determinations through the use of an adsorptive hydrophobic resin
Author(s) -
Huang Y.,
Mechref Y.,
Tian J.,
Gong H.,
Lennarz W. J.,
Novotny M. V.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20000730)14:14<1233::aid-rcm16>3.0.co;2-p
Subject(s) - chemistry , chromatography , mass spectrometry , oligosaccharide , sample preparation , desorption , analytical chemistry (journal) , adsorption , organic chemistry
A simple microadsorption technique is described to remove detergent additives from oligosaccharide samples before their mass spectrometric analysis. The described methodology has been validated with submicrogram quantities of contaminated glycoproteins. This procedure is applicable to investigating minute quantities of glycans in both the positive‐ and negative‐ion mode of matrix‐assisted laser desorption/ionization mass spectrometry. Copyright © 2000 John Wiley & Sons, Ltd.