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Analysis of urinary nucleosides. I. Optimisation of high performance liquid chromatography/electrospray mass spectrometry
Author(s) -
Dudley Edward,
ElSharkawi Salah,
Games David E.,
Newton Russell P.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20000730)14:14<1200::aid-rcm10>3.0.co;2-i
Subject(s) - chemistry , chromatography , mass spectrometry , high performance liquid chromatography , electrospray ionization , electrospray , direct electron ionization liquid chromatography–mass spectrometry interface , analytical chemistry (journal) , mass spectrum , protonation , ion , ionization , chemical ionization , organic chemistry
In order to optimise the analysis of urinary nucleosides by high performance liquid chromatography/mass spectrometry (HPLC/MS), the HPLC separation of these compounds was performed at different ‘flow rates’ and 0.2mL/min was found to give both a better separation and ionisation. The ionisation conditions were optimised to give the best intensity of the molecules quasi‐molecular ions. The ion distribution profile and ionisation in both positive and negative mode were examined and the detection of the protonated molecule in positive mode chosen for further analysis. The limits of detection of the method developed are reported and representative LC/MS and LC/MS/MS spectra shown. Typical urinary nucleoside chromatograms are presented. Copyright © 2000 John Wiley & Sons, Ltd.