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Electrospray tandem mass spectrometry in the rapid identification of α‐chain haemoglobin variants †
Author(s) -
Rai Dilip K.,
Alvelius Gunvor,
Landin Britta,
Griffiths William J.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20000730)14:14<1184::aid-rcm7>3.0.co;2-s
Subject(s) - chemistry , tandem mass spectrometry , electrospray mass spectrometry , mass spectrometry , electrospray , chromatography , isoelectric focusing , population , identification (biology) , protein mass spectrometry , computational biology , biochemistry , demography , sociology , enzyme , botany , biology
The potential use of electrospray tandem mass spectrometry in the rapid characterisation of haemoglobin variants found in the Swedish population has been assessed. Analysis times of the order of 5 –10 min were routinely achieved, and identification of variants using mass spectrometry as the sole analytical technique was possible. However, additional information, readily available from isoelectric focusing experiments, made identification simpler and more secure. In the present communication we report on the identification of the α‐chain variants, Hb Russ, Hb Le Lamentin and Hb Q‐Iran. The identifications were confirmed by the use of nucleotide sequencing techniques. Copyright © 2000 John Wiley & Sons, Ltd.