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Rapid determination of chlorogenic acid and related compounds in sunflower seeds by high‐performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry
Author(s) -
Aramendía Maria Angeles,
García Isabel María,
Lafont Fernando,
Lizaso Jesús,
Marinas Jose María,
Urbano Francisco José
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/1097-0231(20000630)14:12<1019::aid-rcm981>3.0.co;2-9
Subject(s) - chemistry , atmospheric pressure chemical ionization , chromatography , chlorogenic acid , mass spectrometry , high performance liquid chromatography , chemical ionization , sunflower , extraction (chemistry) , liquid chromatography–mass spectrometry , ionization , analytical chemistry (journal) , organic chemistry , ion , mathematics , combinatorics
High‐performance liquid chromatography (HPLC) in combination with atmospheric pressure chemical ionization mass spectrometry (APcI‐MS) was applied to the determination of the phenolic fraction found in methanolic extracts of sunflower seeds (mainly chlorogenic acid and derived compounds). These extracts were directly separated by HPLC and detected by both negative and positive APcI‐MS. Abundant structural information about these compounds can be obtained even at low extraction cone voltages. This method has been shown to be a rapid and effective method for the analysis of crude extracts from sunflower seeds. © 2000 John Wiley & Sons, Ltd.