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M R 77 KDA factor derived from fibroblasts stimulates the invasion ability of breast‐cancer cells
Author(s) -
Ikeda Katsumi,
Yashiro Masakazu,
Sawada Tetsuji,
Hato Fumihiko,
Hasuma Tadayoshi,
Ishikawa Tetsuro,
Chung Kosei HirakawaY.S.
Publication year - 2001
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/1097-0215(200102)9999:9999<::aid-ijc1175>3.0.co;2-f
Subject(s) - cancer research , breast cancer , medicine , pathology , biology , oncology , cancer
Breast‐cancer cells frequently invade into the skin. However, few reports have described the mechanisms responsible for this invasion. In this study, we investigated the effects of skin fibroblasts on the invasion ability of breast‐cancer cells, using a modification of Boyden's chamber method, and purified an invasion‐stimulating factor from fibroblasts. Conditioned medium of skin fibroblasts, DF‐1, significantly stimulated the invasion ability of breast‐cancer cells, OCUB‐1. The invasion‐stimulating factor produced from skin fibroblasts was then partially purified and characterized. The soluble invasion‐stimulating factor of fibroblasts was a protein of 77 kDa, as calculated by SDS‐PAGE. Although TGF‐α and bFGF stimulated the invasion of OCUB‐1, no inhibition of the 77 kDa factor was achieved with neutralizing antibodies against TGF‐α and bFGF. These findings suggest that the 77 kDa factor derived from skin fibroblasts might be unique, might play an important role in invasion to the skin and might explain the frequent skin invasion in breast carcinoma. © 2001 Wiley‐Liss, Inc.