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p53‐mediated negative regulation of stathmin/Op18 expression is associated with G 2 /M cell‐cycle arrest
Author(s) -
Johnsen John Inge,
Aurelio Oscar N.,
Kwaja Zeenat,
Jörgensen Gunn E.,
Pellegata Natalia S.,
Plattner Rina,
Stanbridge Eric J.,
Cajot JeanFrançois
Publication year - 2000
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/1097-0215(20001201)88:5<685::aid-ijc1>3.0.co;2-z
Subject(s) - stathmin , cell cycle , cell cycle checkpoint , microbiology and biotechnology , biology , cancer research , medicine , cell , microtubule , genetics
Utilizing the technique of differential display of mRNA, we have identified p53 ‐responsive genes that are transcriptionally up‐ or down‐regulated as cells enter growth arrest. One gene that was down‐regulated, pong16, was found to be identical to stathmin/Op18, a protein involved in the regulation of microtubule dynamics. Evidence that p53 is directly or indirectly involved in negative regulation of stathmin/Op18 expression includes the following: (i) p53‐mediated growth inhibition is associated with repression of stathmin/Op18 expression following serum stimulation, (ii) reporter gene assays revealed p53‐mediated repression of stathmin/Op18 promoter activity and (iii) constitutive over‐expression of stathmin/Op18 bypasses a p53‐mediated G 2 /M arrest in the cell cycle. These results suggest that p53‐mediated negative regulation of stathmin/Op18 plays an important role in cell‐cycle control. Int. J. Cancer 88:685–691, 2000. © 2000 Wiley‐Liss, Inc.