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Comparative genomic hybridization of fine needle aspirates from breast carcinomas
Author(s) -
Bürki Nicole G.,
Caduff Rosmarie,
Walt Heinrich,
Moll Carlo,
Pejovic Tanja,
Haller Urs,
Ward David C.
Publication year - 2000
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/1097-0215(20001115)88:4<607::aid-ijc14>3.0.co;2-3
Subject(s) - comparative genomic hybridization , karyotype , chromosome , pathology , metaphase , fluorescence in situ hybridization , biology , breast cancer , cytogenetics , cancer , dna , cancer research , gene , medicine , genetics
Detailed knowledge of chromosomal aberrations in a specific tumor may facilitate the development of individually tailored chemotherapy, hormone or gene therapy. Unfortunately, karyotype analysis requires living cells and is complicated by the low number of good metaphase spreads obtained. Comparative genomic hybridization (CGH), however, is capable of detecting and mapping genome‐wide amplifications and deletions using an equimolar mixture of normal and tumor cell DNA. We show here that even the few cells from a fine needle aspirate of a tumor are sufficient for a direct CGH assay, independent of DNA amplification. Ten primary breast cancers were analyzed by CGH. A fresh frozen fine needle aspirate and a formalin‐fixed and paraffin‐embedded section were used for each tumor. Metaphases from each CGH reaction were imaged, and a sum ratio profile was determined for every chromosome. The ratio profiles of DNA isolated from the 2 material sources were then compared. Fine needle aspirates and the paraffin‐embedded material of a single tumor yielded the same fluorescence ratio profiles, albeit with slightly different confidence intervals. Different tumors showed a variety of aberrations. The most frequently observed changes were 1q+, 8q+, 14q–, 16p+, 16q–, 17p–, 17q+, 19q+, 20q+, 21q– and 22q–. The ability of CGH to assess chromosomal changes in breast cancer from fine needle aspirates could facilitate genetic evaluation of tumors prior to surgery. Int. J. Cancer 88:607–613, 2000. © 2000 Wiley‐Liss, Inc.