Premium
A HER2/NEU‐derived peptide, a K d ‐restricted murine tumor rejection antigen, induces HER2‐specific HLA‐A2402‐restricted CD8 + cytotoxic T lymphocytes
Author(s) -
Ikuta Yasushi,
Okugawa Toshiharu,
Furugen Reiko,
Nagata Yasuhiro,
Takahashi Yoshiyuki,
Wang Lijie,
Ikeda Hiroaki,
Watanabe Masato,
Imai Shigemi,
Shiku Hiroshi
Publication year - 2000
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/1097-0215(20000815)87:4<553::aid-ijc15>3.0.co;2-8
Subject(s) - cytotoxic t cell , ctl* , cd8 , biology , microbiology and biotechnology , antigen , human leukocyte antigen , epitope , immunology , cancer research , in vitro , biochemistry
We have identified an H‐2K d ‐binding peptide, HER2p780 (PYVSRLLGI), derived from murine HER2/neu (HER2), that can induce HER2‐specific murine cytotoxic T lymphocytes (CTL). Weekly vaccination of BALB/c mice by syngeneic dendritic cells pulsed with HER2p780 peptide, entirely common to murine and human HER2, suppressed growth of pretransplanted HER2‐expressing syngeneic tumors. A HER2‐expressing human cancer cell line SKOV3 transfected with murine H‐2K d cDNA could also be lysed by HER2p780‐specific murine CTLs, indicating that human HER2‐expressing cancer cells can process and present the cognate peptide in the context of H‐2K d . Since H‐2K d and HLA‐A2402 molecules have similar anchor motifs, the possibility of inducing HER2‐specific CTL activity with HER2p780 in HLA‐A2402 individuals was examined. CD8 + CTL clones specific for HER2‐expressing cancer cell lines were established from peripheral blood lymphocytes with HLA‐A2402 by repeatedly sensitizing with peptide‐pulsed autologous dendritic cells as well as peripheral blood mononuclear cells. Detailed analysis of their specificity revealed that the cytotoxicity of CTL clones is specific for the cognate peptide with HLA‐A2402 restriction. The results suggest that HER2p780 is a unique peptide that may function as a tumor rejection antigen peptide in HLA‐A2402 individuals, as it was directly proven here to function in a murine tumor system. Int. J. Cancer 87:553–558, 2000. © 2000 Wiley‐Liss, Inc.