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Prognostic implications of microsatellite genotypes in gastric carcinoma
Author(s) -
Choi SangWook,
Choi JungRan,
Chung YeunJun,
Kim KyoungMee,
Rhyu MunGan
Publication year - 2000
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/1097-0215(20000720)89:4<378::aid-ijc10>3.0.co;2-t
Subject(s) - loss of heterozygosity , microsatellite instability , microsatellite , biology , genotype , cancer , univariate analysis , carcinoma , medicine , pathology , genetics , multivariate analysis , allele , gene
Microsatellite alterations such as loss of heterozygosity (LOH) and microsatellite instability (MSI) are observed in most (70% to 80%) gastric carcinomas. To determine whether the microsatellite genotypes are correlated with clinicopathological features, 118 patients with gastric carcinomas were examined by using polymorphic microsatellite markers for LOH on 5 gastric cancer‐associated chromosome arms and non‐polymorphic BAT markers for MSI. Microsatellite genotypes were categorized as high‐frequency MSI (MSI‐H), high‐level LOH (LOH‐H), low‐level LOH (LOH‐L) and LOH non‐detectable (LOH‐N). A significant fraction of the MSI‐H, LOH‐H and LOH‐L types was observed in intestinal‐type gastric carcinomas, whereas the LOH‐N type was highly associated with diffuse‐type tumors ( p = 0.00162). There was a close relationship between microsatellite genotype and TNM (tumor‐node‐metastasis) stage ( p = 0.001). Univariate analysis showed that patients of LOH‐H or LOH‐N types and those of MSI‐H or LOH‐L types correlated with poor and favorable survival, respectively, not only in all tumor stages ( p = 0.0001) but also in stages II and III ( p = 0.0271). It is likely that the major genotypes of gastric carcinomas can be placed into at least 4 microsatellite categories, thus allowing the construction of a comprehensive genetic classification useful for the prediction of diverse clinical courses. Int. J. Cancer 89:378–383, 2000. © 2000 Wiley‐Liss, Inc.