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SSA/RO52 gene and expressed sequence tags in an 85 kb region of chromosome segment 11p15.5
Author(s) -
Kim YoungChul,
Cao Youjia,
Pitterle Diana M.,
O'Briant Kathy C.,
Bepler Gerold
Publication year - 2000
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/1097-0215(20000701)87:1<61::aid-ijc9>3.0.co;2-r
Subject(s) - biology , exon , genetics , gene , allele , microbiology and biotechnology , tumor suppressor gene , lung cancer , single strand conformation polymorphism , southern blot , chromosome , carcinogenesis , pathology , medicine
Frequent allelic loss in lung cancer has been described in a region on chromosome segment 11p15.5 (LOH11A). The region is approximately 650 kb in size and flanked by the markers D11S988 centromeric and D11S860 telomeric. Clinical and cell biological studies suggest that it contains a gene associated with metastatic tumor spread. One of the genes identified within this region is SSA/Ro52, which has a RING finger domain and may be involved in gene regulation. We studied this gene for mutations using SSCP analysis and for expression using RT‐PCR and Western blotting on lung cancer cell lines and tumor–normal tissue pairs. No mutations and no differences in mRNA or protein expression between tumor tissue and normal tissue pairs were identified. We discovered a novel polymorphic site (SSA44C/T) within exon 1 of this gene. Among 141 primary lung cancers, allelic loss was observed in 16% of informative cases. Our analyses excluded SSA/Ro52 as a tumor‐suppressor gene in lung cancer and newly defined the centromeric border of the LOH11A region from D11S988 previously to SSA44C/T. This reduced the region of the putative suppressor gene to 460 to 485 kb. A significant difference ( p = 0.01) in the frequency of alleles for this polymorphism between Caucasians and African‐Americans was observed. The “T” allele frequency was 0.12 in Caucasians and 0.23 in African‐Americans. A genomic Eco RI map over 85 kb surrounding the SSA/Ro52 gene was constructed, and 4 expressed sequence tags were identified by sequencing and studied. Int. J. Cancer 87:61–67, 2000. © 2000 Wiley‐Liss, Inc.