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Gene expression profiling of malignant mesothelioma cell lines: cDNA array study
Author(s) -
Kettunen Eeva,
Nissén AnnaMaria,
Ollikainen Tiina,
Taavitsainen Matti,
Tapper Johanna,
Mattson Karin,
Linnainmaa Kaija,
Knuutila Sakari,
ElRifai Wa′el
Publication year - 2001
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/1097-0215(200002)9999:9999<::aid-ijc1094>3.0.co;2-m
Subject(s) - complementary dna , biology , gene , microbiology and biotechnology , gene expression profiling , cell culture , gene expression , cyclin d2 , cell , cell cycle , cell growth , cancer research , cyclin d1 , genetics
To reveal genes relevant for malignant mesothelioma (MM), we carried out cDNA array experiments on 4 MM cell lines and 2 primary mesothelial cell cultures established from pleural fluid of non‐cancer patients. Human cancer gene filters including 588 genes were used for the cDNA array experiments. Our study revealed 26 over‐expressed genes that play a role in the regulation of cell fate, cell cycle, cell growth and DNA damage repair and 13 under‐expressed genes encoding growth factors, receptors and proteins involved in cell adhesion, motility and invasion to be common to 3 or 4 MM cell lines. We confirmed the cDNA array results using RT‐PCR for 5 of the over‐expressed genes and for 3 of the under‐expressed genes. Our study presents gene expression profiles in MM cell lines and shows the involvement of several genes, such as those encoding JAGGED1, ser/thr protein kinase NIK, Ku80 and cyclin D2, novel in MM. © 2001 Wiley‐Liss, Inc.