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Association between CagA + Helicobacter pylori infection and p 53, bax and transforming growth factor‐β‐RII gene mutations in gastric cancer patients
Author(s) -
Deguchi R.,
Takagi A.,
Kawata H.,
Inoko H.,
Miwa T.
Publication year - 2001
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/1097-0215(200002)9999:9999<::aid-ijc1088>3.0.co;2-b
Subject(s) - caga , helicobacter pylori , cancer , gastroenterology , antibody , biology , carcinogenesis , medicine , gene , immunology , virulence , genetics
We assessed the possible association between CagA + Helicobacter pylori infection and gastric carcinogenesis in gastric cancer patients. Gastric biopsy specimens were obtained from 64 patients with gastric cancer and were histologically classified into intestinal and diffuse types. H. pylori infection was determined by cultivation, flaA ‐PCR and serum antibody against CagA. p53 , BAX and transforming growth factor‐β‐RII ( TGFβ‐RII ) gene mutations were analyzed by PCR‐SSCP and direct sequencing. Intestinal and diffuse types of cancer were detected in 45 and 19 patients, respectively. H. pylori infection was found in 55 (85.9%) of 64 patients. There was no significant difference in H. pylori positivity between intestinal and diffuse types. However, the CagA antibody was positive in 15 (78.9%) of 19 patients with the diffuse type and in 22 (48.9%) of 45 patients with the intestinal type ( p = 0.030). Among the 55 H. pylori ‐positive cases, 11 (29.7%) of the 37 patients in the CagA + group were found to have p53 alterations, compared with 2 (11.1%) in the 18 CagA ‐ group ( p = 0.182). Moreover, among the 64 gastric cancer patients, p53 alterations were more frequently found in the CagA + group (29.7%) than in the H. pylori ‐positive CagA ‐ and H. pylori ‐negative groups (7.4%; p = 0.033). BAX gene mutations were found in 19 (29.7%) of 64 patients and there was no relationship among CagA seropositivity, cancer stages and histopathological phenotypes. In contrast, the TGFβ‐RII gene mutation was only detected in one CagA ‐ patient. The results suggest that CagA + H. pylori infection may have an important role in the development of gastric cancer patients with p53 mutations © 2001 Wiley‐Liss, Inc.