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Non‐random chromosomal rearrangements in pancreatic cancer cell lines identified by spectral karyotyping
Author(s) -
Sirivatanauksorn Vorapan,
Sirivatanauksorn Yongyut,
Gorman Patricia A.,
Davidson Joanne M.,
Sheer Denise,
Moore Patrick S.,
Scarpa Aldo,
Edwards Paul A.W.,
Lemoine Nicholas R.
Publication year - 2000
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/1097-0215(200002)9999:9999<::aid-ijc1049>3.0.co;2-c
Subject(s) - karyotype , cdkn2a , biology , fluorescence in situ hybridization , kras , comparative genomic hybridization , cytogenetics , molecular cytogenetics , pancreatic cancer , breakpoint , genetics , cancer research , cancer , chromosome , microbiology and biotechnology , gene , colorectal cancer
The molecular events involved in pancreatic cancer are becoming increasingly well characterized, with mutations in the dominant oncogene KRAS and the tumour suppressor genes TP53 , CDKN2A and MADH4 being typically observed. However, other genetic abnormalities remain to be identified and molecular cytogenetics may be useful to detect chromosomal loci involved in recurrent rearrangements. We have used spectral karyotyping to characterize cytogenetic aberrations in a panel of 20 human pancreatic carcinoma cell lines and confirmed their identities by dual and triple color fluorescence in situ hybridization. The most common partial or whole‐arm gains involved 5p, 7q, 12p, 1q, 7p, 5q, 9p, 9q and 11p. The most common partial or whole‐arm losses affected 9p, 11q, 18q, 3p, 2q and 1p, as well as the short arms of the acrocentric chromosomes. Spectral karyotyping allowed us to identify a number of recurrent structural aberrations, all of them unbalanced: most frequently i(5)(p10), del(11)(q23), i(12)(p10), i(1)(q10), del(7)(q22) and del(10)(p11). Spectral karyotyping mapped the complex aberrations occurring in pancreatic cancer cell lines and identified non‐random patterns of chromosomal rearrangement. This comprehensive characterization should be useful to direct future investigation. The observation that loss at 11q and gains at 5p with i(5)(p10) and 12p with i(12)(p10) are more frequent changes than previously reported would justify more intensive investigation of these chromosomal regions. © 2001 Wiley‐Liss, Inc.