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Low frequency of apoptosis in Epstein‐Barr virus‐associated gastric carcinoma with lymphoid stroma
Author(s) -
Ohfuji Satoshi,
Osaki Mitsuhiko,
Tsujitani Shunichi,
Ikeguchi Masahidc,
Sairenji Takeshi,
Ito Hisao
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/1097-0215(19961211)68:6<710::aid-ijc2910680602>3.0.co;2-6
Subject(s) - tunel assay , stroma , terminal deoxynucleotidyl transferase , apoptosis , pathology , biology , carcinoma , lymphatic system , epstein–barr virus , virus , gastric carcinoma , immunohistochemistry , medicine , cancer , immunology , biochemistry , genetics
In order to understand the character of Epstein‐Barr virus (EBV)‐associated gastric carcinoma with lymphoid stroma (GCLS), we examined cell proliferation and death in comparison with gastric carcinoma without prominent lymphoid stroma (conventional gastric carcinoma). The EBV‐encoded small RNA I (EBER‐I) and apoptotic cells were examined by in situ hybridization (ISH) and by terminal deoxynucleotidyl tramferare (TdT)‐mediated dUTP‐biotin nick end‐labeling (TUNEL), respedvely, on formalin‐fmed paraffin‐embedded surgical speci‐mens from 22 GCLSs and 23 conventional gastric carcinomas. lmmunostaining was performed for the detection of Ki‐67 antigen (Ki‐67), P53 protein (P53) and bcl‐2 oncoprotein (BCL‐2). The percentages of apoptotic and Ki‐67‐positive cells were expressed as apoptotic indices (Al) and Ki‐67 labeling indices (KI), respectively. EBER‐ I was detected in I9 (86%) GCLSs in contrast to none in conventional carcinomas. Mean Al was 1.8 ± 0.6 in the EBER‐ I ‐positive GCLSs and 3.3 ± 1.7 in the conventional gastric carcinomas, the value being significantly lower in the former (p < 0.01). Mean KI was 40.0 ± 10.8 in the GCLSs and 48.5 ± 9.1 in the conventional gastric carcinomas, the value being significantly lower in the former (p < 0.05). P53 expression showed no significant difference between the 2 categories of carcinoma.