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Stimulatory effects of pleural fluids from mesothelioma patients on CD44 expression, hyaluronan production and cell proliferation in primary cultures of normal mesothelial and transformed cells
Author(s) -
Teder Priit,
Versnel Marjan A.,
Heldin Paraskevi
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/1097-0215(19960729)67:3<393::aid-ijc2910670302>3.0.co;2-y
Subject(s) - mesothelial cell , mesothelioma , mesothelium , cd44 , cancer research , pathology , receptor , chemistry , medicine , biology , cell , biochemistry
Cell lines established from human malignant mesotheliomas, but not from normal mesothelial cells, have been shown possess hyaluronan receptors, and to secrete factors that stimulate hyaluronan production by fibroblasts and normal mesothelial cells. In the present study we investigated the generality of this observation, namely the presence of hyaluro nan receptors and factors which induce stimulation of hyaluronan synthesis in primary mesothelioma and mesothelial cell cultures. Functionally active hyaluronan‐binding sites on the surface of malignant mesothelioma cells in primary cultures, established from pleural effusions of 3 different patients, were demonstrated using 3H‐hyaluronan. Primary cultures of normal mesothelial cells from non‐mesothelioma effusions did not exhibit any binding ability. Pleural fluids from mesothelioma patients both stimulated hyaluronan synthesis and promoted proliferation of normal mesothelial cells to a larger extent than non‐mesothelioma fluids. The hyaluronan‐stimulatory activity was only slightly neutralized by antibodies against PDGF‐BB TGF‐8; antibodies against bFGF had no effect. Although the concentration of hyaluronan was much higher in pleural fluids from mesothelioma than from non‐mesothelioma patients, molecular weight was almost the same. The hyaluronan‐binding capacity of early‐passage mesothelioma cells derived from pleural effusions can be an additional marker, in combination with other diagnostic tools, to distinguish between mesothelioma and mesothelial cells.