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Dissection of the odontoblast differentiation process in vitro by a combination of FGF1, FGF2, and TGFβ1
Author(s) -
Unda FernandoJosé,
Martín Amaya,
Hilario Enrique,
BègueKirn Catherine,
Ruch JeanVictor,
Aréchaga Juan
Publication year - 2000
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/1097-0177(200007)218:3<480::aid-dvdy1011>3.0.co;2-o
Subject(s) - odontoblast , alkaline phosphatase , microbiology and biotechnology , biology , secretion , extracellular matrix , cellular differentiation , biochemistry , pathology , enzyme , pulp (tooth) , medicine , gene
Dental papillae (DP) isolated from first lower molars of 17‐day‐old mouse embryos were cultured in the presence of combinations of the following growth factors: FGF1, FGF2, and TGFβ1. After 6 days in culture, only the DP treated with FGF1+TGFβ1 contained differentiated odontoblast‐like cells at the periphery of the explants, and these cells secreted extracellular matrix similar to predentin. Surprisingly, treatments with FGF2+TGFβ1 induced cell polarization at the surface of the explants but no matrix secretion was observed. Electron microscopy and histochemical analysis of odontoblast markers showed that differentiated cells induced by FGF1+TGFβ1 exhibited cytological features of functional odontoblasts with matrix vesicle secretion and mineral formation, positive alkaline‐phosphatase activity, and type‐I collagen production. DP cultured in the presence of FGF2+TGFβ1 showed cell polarization and long and thin cell processes containing matrix vesicles; however, type‐I collagen secretion was not detected and alkaline‐phosphatase activity was completely inhibited. Our results indicate that, in our culture system, exogenous combinations of FGF1, FGF2, and TGFβ1 interact with preodontoblasts and induce cell polarization or differentiation, which can be studied separately in vitro. Thus, FGF1 and TGFβ1 do have a synergic effect to promote morphological and functional features of differentiated odontoblasts whereas FGF2 seems to modulate TGFβ1 action, causing morphological polarization of preodontoblasts but limiting the functional activity of these cells in terms of type‐I collagen secretion and alkaline‐phosphatase activity. Dev Dyn 2000;218:480–489. © 2000 Wiley‐Liss, Inc.