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Interaction of Frizzled 7 and Dishevelled in Xenopus
Author(s) -
Medina Araceli,
Steinbeisser Herbert
Publication year - 2000
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/1097-0177(2000)9999:9999<::aid-dvdy1017>3.0.co;2-9
Subject(s) - frizzled , dishevelled , wnt signaling pathway , lrp6 , biology , lrp5 , xenopus , microbiology and biotechnology , receptor , signal transduction , genetics , gene
Frizzled proteins act as putative Wnt receptors and depending on Wnt/Frizzled interactions distinct intracellular pathways can be activated. The canonical Wnt pathway, triggered by Wnt‐1‐type ligands, is the best characterized and involves the activation of the cytoplasmic protein Dishevelled (Dsh). The Xenopus frizzled 7 receptor ( Xfz 7) can act in the canonical (Wnt‐1‐type) as well as in a non‐canonical Wnt pathway which involves the activation of protein kinase C (PKC). In order to analyze the interaction between Xfz7 and Xdsh protein, we tested the effect of overexpression of Xfz 7 on the subcellular distribution of Xdsh‐myc protein. We demonstrate that Xfz7 can recruit Xdsh‐myc to the plasma membrane and target genes of the Wnt‐1‐type pathway such as siamois and nodal related 3 ( Xnr ‐3) are only activated in the presence of exogenous Xdsh‐myc. Xfz7 in combination with Xwnt‐8b, however, is able to induce siamois even in the absence of Xdsh‐myc. Similar results were obtained after expression of Human frizzled 5 ( Hfz 5) together with Xwnt ‐5 a but this receptor‐ligand combination recruits Xdsh‐myc only very poorly to the membrane. These results suggests that the endogenous Xdsh pool and exogenous Xdsh‐myc differ in their ability to be recruited by Frizzled receptors. © 2000 Wiley‐Liss, Inc.