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Molecular detection of metastatic retinoblastoma cells by reverse transcription polymerase reaction for interphotoreceptor retinoid‐binding protein MRNA
Author(s) -
Yamashita Nobuko,
Nishiuchi Ritsuo,
Oda Megumi,
Tomiyama Yoshie,
Eguchi Naohiro,
Endo Chie,
Manki Akira,
Seino Yoshiki
Publication year - 2001
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(20010415)91:8<1568::aid-cncr1167>3.0.co;2-v
Subject(s) - retinoblastoma , bone marrow , reverse transcription polymerase chain reaction , microbiology and biotechnology , messenger rna , protein subunit , neuroblastoma , gene expression , medicine , reverse transcriptase , gene , transcription (linguistics) , pathology , cancer research , polymerase chain reaction , biology , cell culture , biochemistry , linguistics , philosophy , genetics
BACKGROUND In the current study, the authors report a 4 year old girl with disseminated retinoblastoma. To find sensitive and specific molecular markers for detection of retinoblastoma cells in blood and marrow, the authors evaluated three photoreceptor‐associated gene transcripts by using reverse transcription polymerase chain reaction (RT‐PCR). METHOD Samples of bone marrow and blood were obtained from healthy donors and the patient. RT‐PCR was performed to detect the cone α′‐subunit of cGMP phosphodiesterase (cone α′‐PDE), the rod β‐subunit of cGMP (rod β‐PDE), and the interphotoreceptor retinoid‐binding protein (IRBP) gene transcript in RNA extracted from the samples. RESULTS While no expression of rod β‐PDE or IRBP was detected in any of the normal samples, expression of cone α′‐PDE was detected in two out of seven normal marrow samples. Expression of rod β‐PDE was not detected in the patient samples. Expression of IRBP was detected in the patient samples obtained from iliac bone marrow before intensive chemotherapy but not thereafter. CONCLUSION RT‐PCR for IRBP was a useful method for detecting metastatic retinoblastoma cells as well as for evaluating the therapeutic effects of treatment in this particular case. Cancer 2001;91:1568–73. © 2001 American Cancer Society.