Premium
Granulocyte colony‐stimulating factor (G‐CSF) receptor gene expression of ovarian carcinoma does not correlate with G‐CSF caused cell proliferation
Author(s) -
Brandstetter Thomas,
Ninci Elena,
Bettendorf Herta,
Perewusnyk Gloria,
Stolte Jens,
Herchenbach Dieter,
Sellin Dieter,
Wagner Edgar,
Köchli Ossi R.,
Bauknecht Thomas
Publication year - 2001
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(20010401)91:7<1372::aid-cncr1141>3.0.co;2-e
Subject(s) - cell culture , cancer research , ovarian carcinoma , ovarian cancer , cell growth , hematopoietic growth factor , medicine , pathology , granulocyte colony stimulating factor , biology , carcinoma , haematopoiesis , cancer , stem cell , chemotherapy , microbiology and biotechnology , genetics
BACKGROUND Recombinant human granulocyte colony‐stimulating factor (rhG‐CSF) is a growth factor commonly used to avoid leukopenia after chemotherapy. Endogenous G‐CSF is produced by macrophages and granulocytes that infiltrate tumors. It has been reported that rhG‐CSF stimulates the proliferation of several cell lines as well as bladder carcinoma cells. Conversely, in some hematopoietic cell lines such as U‐937, WEHI‐3B, and K‐562 no effect or in some cases a differentiation pattern was found. Moreover, the role of rhG‐CSF on the proliferation of solid tumors is not well understood. METHODS In this study, 10 ovarian carcinoma biopsies were characterized for the presence of G‐CSF and G‐CSF receptor by reverse transcription–polymerase chain reaction (RT‐PCR) and immunohistochemical analysis. Proliferation was analyzed by ATP viability assays. RESULTS Performing RT‐PCR, these biopsies and four ovarian carcinoma cell lines were analyzed for endogenous G‐CSF production, which was found in some biopsies and in all cell lines. Despite the presence of the G‐CSF receptor in all biopsies and cell lines, no proliferation was found after rhG‐CSF incubation of the cell lines or the tumor samples for 3 and for 6 days, respectively. CONCLUSIONS Summarizing the authors' in vitro studies, rhG‐CSF does not affect the proliferation of ovarian carcinoma cells in vitro. Cancer 2001;91:1372–83. © 2001 American Cancer Society.