Premium
Increased expression of laminin‐5 and its prognostic significance in lung adenocarcinomas of small size
Author(s) -
Moriya Yasumitsu,
Niki Toshiro,
Yamada Tesshi,
Matsuno Yoshihiro,
Kondo Haruhiko,
Hirohashi Setsuo
Publication year - 2001
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(20010315)91:6<1129::aid-cncr1109>3.0.co;2-c
Subject(s) - laminin , pathology , medicine , stromal cell , lymphovascular invasion , adenocarcinoma , cancer research , cancer , biology , cell , metastasis , genetics
BACKGROUND Laminin‐5 plays an important role in cell migration during tissue remodeling and tumor invasion. METHODS The authors studied the expression of laminin‐5 immunohistochemically in 102 cases of small‐sized lung adenocarcinoma (maximum dimension ≤ 2 cm) using a monoclonal antibody against the laminin γ2 chain, and they also investigated the associations of laminin‐5 with clinicopathologic characteristics. Prognostic significance of increased laminin‐5 expression was evaluated using the Kaplan–Meier method and the Cox proportional hazard model. RESULTS Overall, laminin‐5 expression was observed in 82 cases (80.4%): 7 of 18 (38.9%) bronchioloalveolar carcinomas and 75 of 84 (89.3%) invasive adenocarcinomas. Laminin‐5 was preferentially localized in the cytoplasm of tumor cells at the tumor–stromal interface, where budding or dissociation of cancer cells was frequently observed. Overexpression of laminin‐5 (24 cases, 23.5%) was associated with vascular invasion ( P = 0.021) and stromal fibroblastic reaction ( P = 0.005) but not with nodal involvement, lymphatic invasion, or pleural invasion. Survival analysis revealed that overexpression of laminin‐5 was associated with shorter patient survival ( P = 0.0027 by log rank test). On multivariate analysis, overexpression of laminin‐5 was an independent prognostic factor ( P = 0.030), as were nodal involvement ( P < 0.0001), vascular invasion ( P = 0.047), and lymphatic invasion ( P = 0.0047) in a whole cohort of patients. Moreover, when patients with Stage I (International Union Against Cancer [UICC] staging system) disease were considered in multivariate analysis, overexpression of laminin‐5 was the only significant prognostic factor ( P = 0.022), whereas vascular invasion had a marginally significant impact ( P = 0.07) on patient survival. CONCLUSIONS The authors' results showed that laminin‐5 is frequently expressed by cancer cells at the invasive front of lung adenocarcinoma. The study concluded that overexpression of laminin‐5 may be a useful prognostic factor in patients with small‐sized lung adenocarcinoma, especially in Stage I cases. Cancer 2001;91:1129–41. © 2001 American Cancer Society.