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The cytomorphology of ocular surface squamous neoplasia by using impression cytology
Author(s) -
Nolan Glenda R.,
Hirst Lawrence W.,
Bancroft B. Josephine
Publication year - 2001
Publication title -
cancer cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(20010225)93:1<60::aid-cncr9008>3.0.co;2-5
Subject(s) - histopathology , medicine , cytology , pathology , intraepithelial neoplasia , hyperkeratosis , dermatology , carcinoma in situ , squamous intraepithelial lesion , cancer , carcinoma , cervical intraepithelial neoplasia , prostate , cervical cancer
BACKGROUND The term ocular surface squamous neoplasia (OSSN) encompasses conjunctival and corneal intraepithelial neoplasia through to invasive squamous cell carcinoma of the ocular surface. The disease is related to prolonged exposure to solar ultraviolet light and has been proposed as an acquired immune deficiency syndrome–associated tumor. To the authors' knowledge, very few reports describing the cytology of these lesions have been published. METHODS Impression cytology (IC) samples collected from the eyes of patients with a range of ocular surface diseases were available for study. From these, 267 sets of impressions had subsequent histopathology that had been collected within 6 months of the IC, and which indicated the presence of OSSN. The IC from these cases was used to describe the cytomorphology of intraepithelial and invasive OSSN. RESULTS Within the intraepithelial group, keratinized dysplastic cells that often were accompanied by hyperkeratosis, syncytial‐like groupings, and nonkeratinized dysplastic cells were described. Within the invasive group, cases with significant keratinization and an additional group of cases with little keratinization and sometimes also prominent macronucleoli were described. Keratinized cases were the most numerous in both the intraepithelial and invasive groups. A description also was given of a low number of cases with cytology and also subsequent histopathology indicating the presence of intraepithelial OSSN, in the absence of a clinically detectable lesion. CONCLUSIONS This detailed description of the cytomorphology of a high number of cases of OSSN with confirmation by histopathology should assist others with little experience of the cytology of these lesions to examine them with increased confidence. Cancer (Cancer Cytopathol) 2001;93:60–67. © 2001 American Cancer Society.