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DNA ploidy analysis and cell proliferation in congenital sacrococcygeal teratomas
Author(s) -
Krus̆lin Boz̆o,
Vis̆njić Andreja,
C̆izmić Ante,
Tomic̆ić Ivana,
Kos Marina,
Jukić Stanko,
Seiwerth Sven
Publication year - 2000
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(20000815)89:4<932::aid-cncr29>3.0.co;2-0
Subject(s) - proliferating cell nuclear antigen , immature teratoma , pathology , immunostaining , flow cytometry , teratoma , germ cell , biology , immunohistochemistry , proliferation index , sacrococcygeal teratoma , ploidy , cell growth , germ cell tumors , medicine , microbiology and biotechnology , fetus , genetics , pregnancy , chemotherapy , gene
BACKGROUND Congenital sacrococcygeal teratoma is the most common germ cell tumor in infants and children. It usually is diagnosed at birth, is benign, and consists of fully differentiated mature tissues. Congenital sacrococcygeal teratomas (SCTs) also may contain immature tissues, most commonly of neural origin. The proportion of malignant teratomas increases with advancing age, but the relation between mature and immature SCTs is not well understood. Thus, it is very important to determine proliferative activity, DNA ploidy, and DNA index to predict biologic behavior of these tumors. METHODS DNA ploidy and cell proliferation were analyzed by flow cytometry, and the expression of proliferating cell nuclear antigen (PCNA) and Ki‐67 were analyzed immunohistochemically on paraffin embedded tissue. RESULTS All the tumors that were surgically treated within 3 months after birth, including immature teratoma, were diploid. Strongly positive PCNA immunostaining was found in both immature teratomas, and weakly positive PCNA was found in nine cases. Weak positivity for Ki‐67 was observed in 2 cases, and moderate positivity was observed in 6 cases including immature teratomas. CONCLUSION The value of flow cytometry in the prediction of biologic behavior of congenital SCT should be analyzed further. Our results suggest that Ki‐67 and especially PCNA may reflect the proliferative activity of these tumors. Cancer 2000;89:932–7. © 2000 American Cancer Society.