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Radioimmunoassay for plasma glutathione S‐transferase‐pi and its clinical application in gastrointestinal cancer
Author(s) -
Fan KaiChun,
Huang YingCai,
Li ChunHai
Publication year - 1995
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19951015)76:8<1363::aid-cncr2820760811>3.0.co;2-4
Subject(s) - radioimmunoassay , medicine , cancer , colorectal cancer , gastrointestinal cancer , gastroenterology , liver cancer , endocrinology
Background . Plasma acidic glutathione S‐transferase (GST‐pi) concentrations in 135 patients with gastrointestinal cancer were measured by an improved radioimmunoassay (RIA). Methods . Blood was collected into a silicified glass tube containing a specific anticoagulant. The plasma, which was separated rapidly by centrifugation with adequate force, underwent RIA procedures (double antibody and polyethylene glycol separation method). Results . The improved RIA for plasma GST‐pi was specific and sensitive. Plasma GST‐pi in 75% of patients with esophageal cancer, 64.44% of those with gastric cancer, 74.41% of those with colon cancer, 85.19% of those with hepatocellular carcinoma, and 60.00% of those with pancreatic cancer was elevated higher than 13.99 μg/I (mean + 1.96 standard deviation of normal controls). Plasma GST‐pi levels in patients with gastric cancer with Stages I‐II, III and IV disease increased in proper order. Plasma GST‐pi concentrations in 57% of patients with colon cancer decreased to the normal range 14 days after surgery. Plasma GST‐pi levels of patients with recurrent colon cancer were higher than of those with primary colon cancer. Conclusion . The procedure for specimen collection must be critical. Plasma GST‐pi may be useful in diagnosing gastrointestinal cancer and in monitoring its clinical course.