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Atypical fibroxanthoma versus benign and malignant fibrous histiocytoma. A comparative study of their proliferative activity using MIB‐1, DNA flow cytometry, and p53 immunostaining
Author(s) -
Oshiro Yumi,
Fukuda Toshiro,
Tsuneyoshi Masazumi
Publication year - 1995
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19950301)75:5<1128::aid-cncr2820750512>3.0.co;2-h
Subject(s) - atypical fibroxanthoma , immunostaining , pathology , immunohistochemistry , flow cytometry , ki 67 , proliferative index , monoclonal antibody , histology , medicine , monoclonal , antibody , immunology
Background. MIB‐1 was found to be detectable in formalin fixed, paraffin embedded materials with microwave treatment using Ki‐67 monoclonal antibody, and immunostaining has been widely documented as a useful marker of proliferation. Because atypical fibroxanthoma (AFX) is regarded as a fibrohistiocytic tumor with an intermediate potential, the proliferative activity of AFX was compared with that of benign and malignant fibrous histiocytomas. Methods. Thirty‐eight soft tissue tumors including atypical fibroxanthoma (n = 5), benign fibrous histiocytoma (FH) (n = 17) and malignant fibrous histiocytoma (MFH) (n = 16) were examined using immunohistochemistry to determine MIB‐1, DNA flow cytometry, and p53 (PAb 1801) immunostaining. Results. The mean of the MIB‐1 labeling index (MIB‐1 LI), defined as the percentage of positive cells for more than 500 cells, was determined in an increasing order as follows: FH, 3.3 ± 1.8; AFX, 12.2 ± 6.3; and MFH, 21.5 ± 10.2. However, the MIB‐1 LI of each case in AFX was considerably scattered, and the MIB‐1 LI of AFX and MFH overlapped each other. A DNA analysis revealed that the proliferative index (S + G 2 + M fraction) showed no significant correlation with the MIB‐1 LI, and an aneuploid pattern was present in only five (42%) of 12 cases of MFH. p53 positivity was detected in 2 (40%) of 5 cases of AFX and 6 (38%) of 16 cases of MFH. Conclusions. Although AFX shows a lower degree in the MIB‐1 LI than MFH, the MIB‐1 LI shows a limited value in relation to the biologic activity of fibrohistiocytic tumors. Aneuploidy demonstrates a malignant potential in fibrohistiocytic tumors. Cancer 1995;75:1128–34.