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Immunohistochemical detection of bone morphogenetic proteins in bone and soft‐tissue sarcomas
Author(s) -
Yoshikawa Hideki,
Rettig Wolfgang J.,
Lane Joseph M.,
Takaoka Kunio,
Alderman Edward,
Rup Bonita,
Rosen Vicki,
Healey John H.,
Huvos Andrew G.,
GarinChesa Pilar
Publication year - 1994
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19940801)74:3<842::aid-cncr2820740309>3.0.co;2-b
Subject(s) - pathology , immunostaining , immunohistochemistry , bone morphogenetic protein , soft tissue , osteosarcoma , medicine , sarcoma , chondrosarcoma , immunoperoxidase , synovial sarcoma , mesenchymal stem cell , biology , monoclonal antibody , antibody , biochemistry , immunology , gene
Background . Bone morphogenetic proteins (BMPs) are potent inducers of bone formation. Functional and immunohistochemical studies have identified BMPs in a subset of osteosarcomas. In the present study, the authors extend the analysis of BMP expression to other bone and soft tissue sarcomas. Methods . Monoclonal antibody AbH3b2/17 against human BMP‐2 and BMP‐4 was used in avidin‐biotin‐immunoperoxidase assays with frozen sections of bone tumors (71 specimens), soft tissue sarcomas (69 specimens), and normal tissues. Results . Among bone tumors, BMP was detected in osteosarcomas (17 of 29 samples), malignant fibrous histiocytomas (MFHs) (6 of 6), and the spindle cell sarcomatous components of spindle cell (dedifferentiated) chondrosarcomas (4 of 4), but not in conventional chondrosarcomas (0 of 10) or Ewing's sarcomas (0 of 14). Histologic subtypes of osteosarcoma differed for BMP expression, with 8 of 9 fibrohistiocytic, 9 of 13 osteoblastic, and 0 of 5 chondroblastic lesions showing immunostaining. In all BMP‐positive bone tumors, immunostaining was localized in the cytoplasm of primitive mesenchymal cells, with little or no staining in tumor matrix and more mature osteoblastic/chondrocytic cells. Among soft tissue sarcomas, MFHs (11 of 12), liposarcomas (5 of 11), leiomyosarcomas (3 of 9), and malignant schwannomas (3 of 8) showed cytoplasmic BMP immunostaining. Synovial sarcomas (0 of 9), rhabdomyosarcomas (0 of 8), and fibrosarcomas (0 of 7) were BMP‐negative. All normal humantissues tested, including the tissues of a 16‐week‐old fetus, lacked BMP immunoreactivity. Conclusions . Bone morphogenetic protein is expressed in a subset of osteosarcomas, a high proportion of MFHs of bone and soft tissue, and in spindle cell chondrosarcomas. In these tumors, BMP is localized predominantly to the cytoplasm of malignant cells with primitive mesenchymal features; no or little BMP is detected in the more differentiated elements of bone and soft tissue sarcomas, Different histologic types of bone and soft tissue sarcomas may mimic discrete stages of mesenchymal differentiation as defined by BMP expression and histologic criteria.