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Expression of bone morphogenetic proteins in human osteosarcoma. Immunohistochemical detection with monoclonal antibody
Author(s) -
Yoshikawa Hideki,
Rettig Wolfgang J.,
Takaoka Kunio,
Alderman Edward,
Rup Bonita,
Rosen Vicki,
Wozney John M.,
Lane Joseph M.,
Huvos Andrew G.,
GarinChesa Pilar
Publication year - 1994
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19940101)73:1<85::aid-cncr2820730116>3.0.co;2-8
Subject(s) - osteosarcoma , immunohistochemistry , immunostaining , immunoperoxidase , pathology , bone morphogenetic protein , monoclonal antibody , medicine , sarcoma , metastasis , antibody , cancer research , biology , cancer , immunology , gene , biochemistry
Background. Bone morphogenetic proteins (BMP) induce ectopic bone formation in vivo and may play a role in normal bone development. In addition, bone morphogenetic activity, as measured in a bone‐forming assay in immunodeficient, athymic nu / nu mice, is present in a proportion of osteosarcomas; this activity, which may be mediated by BMP, is correlated with a poor prognosis. Methods. The development of a monoclonal antibody against recombinant human BMP‐2, AbH3b2/17, has allowed immunohistochemical localization of BMP in tumor tissues. Cryostat sections of osteosarcomas (21 tumor samples), chondrosarcomas (5 samples), and Ewing's sarcomas of bone (5 samples) were examined with AbH3b2/17 using the avidin‐biotin‐immunoperoxidase method. Results. The authors found AbH3b2/17 immunoreactivity in 12 of the 21 osteosarcoma samples (57% sensitivity) obtained from 20 patients. For one patient, samples of the primary lesion and a subsequent metastasis were tested, and only the latter showed AbH3b2/17 immunore‐activity. The chondrosarcomas and Ewing's sarcomas examined showed no immunoreactivity. In antigen‐positive osteosarcomas, AbH3b2/17 immunostaining was localized predominantly in the cytoplasm of tumor cells. Moreover, the proportion of AbH3b2/17‐reactive cells varied among osteosarcomas with disparate histologic features. Conclusions. The authors identified a rapid and widely applicable method for detecting BMP expression in intact tissues, which may complement and enhance the bone‐forming assay in nu / nu mice as a prognostic procedure in osteosarcomas.