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Characterization of lung squamous cell carcinoma‐derived t‐cell suppressive factor
Author(s) -
Yoshino Ichiro,
Kimura Genki,
Yano Tokujiro,
Miyamoto Masahiro,
Nomoto Kikuo,
Yamada Koji,
Kajii Yasushi,
Onodera Kazukiyo,
Ishida Teruyoshi,
Sugimachi Keizo
Publication year - 1993
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19931015)72:8<2347::aid-cncr2820720810>3.0.co;2-i
Subject(s) - cell culture , cytokine , microbiology and biotechnology , cytotoxicity , trypsin , cancer research , cell growth , transforming growth factor , medicine , biology , immunology , endocrinology , biochemistry , in vitro , genetics , enzyme
Background . The immunosuppressive state of a tumor‐bearing patient is possibly mediated by tumor‐derived factor. In this study, the authors characterized lung squamous cell carcinoma‐derived immunosuppressive factor (LSCF). Methods . The immunosuppressive activity of QG56 (a lung squamous carcinoma cell line)‐derived LSCF was evaluated by the effect of culture supernatant of QG56 on anti‐CD3 monoclonal antibody‐induced T‐cell, response such as proliferation ( 3 H‐thymidine uptake), cytotoxicity ( 51 Cr‐releasing assay), and expression of cytokine mRNA (polymerase chain reaction). The LSCF was partially purified with an ion‐exchange high‐performance liquid chromatography (HPLC) and a gel‐filtration HPLC. Results . The LSCF inhibited proliferation, cytotoxicity, and expression of cytokine mRNA of T‐cells in a dose‐dependent manner. It has a molecular weight of approximately 22 kd, and was sensitive to proteinase K, heating at 60°C, and resistant to treatment with trypsin and pH 3 and 9. These properties appear to be similar to those of transforming growth factor‐β (TGF‐β). However, the activity of the LSCF was not abrogated by anti–TGF‐β sera, and the LSCF did not suppress the proliferation of TGF‐β‐sensitive mink lung cells (Mv1Lu). Conclusions . These data suggest that LSCF may be a novel tumor‐derived immunosuppressive protein factor.