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Dual‐color flow cytometric analysis of megakaryocytic DNA ploidy in the investigation of blastic phase chronic myelogenous leukemia with rearrangement of 3q26
Author(s) -
Kwong Y. L.,
Robertson E. P.,
Lee C. P.,
Chan L. C.
Publication year - 1993
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/1097-0142(19930615)71:12<3882::aid-cncr2820711215>3.0.co;2-0
Subject(s) - chronic myelogenous leukemia , megakaryocytopoiesis , medicine , flow cytometry , leukemia , myeloid , acute myeloblastic leukemia , microbiology and biotechnology , megakaryocyte , cancer research , immunology , biology , haematopoiesis , stem cell
A patient is described with chronic myelogenous leukemia in blastic crisis, in whom numerous circulating platelet fragments and megakaryocytic nuclei were present, with 50% blasts and 50% micromegakaryocytes in the marrow. The blasts expressed myeloid‐associated antigens CD34, CD33, and CD13, whereas the micromegakaryocytes were positive for CD41, CD42b, and CD61. These findings suggested a myeloblastic transformation with a possible megakaryoblastic component. Cytogenetic analysis showed rearrangement of 3q26 in the form of t(2;3) (p13;q26), in addition to t(9; 22) (q34; q11). Dual‐color flow cytometric analysis of DNA content of CD42b‐positive cells showed that the micromegakaryocytes were predominantly 2N, indicating a maturation block before nuclear endoreplication and polyploidization. These findings confirmed a combined myeloblastic and megakaryoblastic transformation. It is concluded that dual‐color flow cytometric DNA analysis is a useful method for the investigation of abnormal megakaryocytopoiesis in hematologic malignancies.